User blog comment:Jo4n/Female Hanji Zoe/@comment-2214433-20130612100205/@comment-10733658-20130612111438

I think that is an excellent point. We need to be cautious, because the evidence I have seen used to argue about Hanji being ambiguous or otherwise different from presented biological sex are things that apply to the majority of the cast.

There is almost zero discussion of sexual orientation or gender identity in the series. I think Ymir is literally the only character to be confirmed, and everyone else merely has assumptions or implications. But we need to error on the side of caution.